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Magnetic Resonance Laboratory in Chemistry, Biology and Medicine

Invited researcher Robert Kaptein
Contract number
11.G34.31.0045
Time span of the project
2011-2013
Head of the laboratory

As of 30.01.2020

45
Number of staff members
45
scientific publications
1
Objects of intellectual property
General information

Name of the project: Magnetic resonance in chemistry, biology and medicine

Strategy for Scientific and Technological Development Priority Level: в


Goals and objectives

Research directions: Study of the mechanism and kinetics of fast chemical reactions with participation of oxidized RNA elements and peptides, applications of parahydrogen-induced polarization of nuclei in heterogeneous catalytic reactions, research of molecular mechanisms of DNA repair and genesis of cataract.

Project objective:

  • Study of structural parameters and reaction capability of short-lived radicals in biological molecules and systems
  • Study of the detailed mechanism of chemical and fermentative DNA repair
  • Development of methods of spin hyperpolarization in chemistry, biology and medicine for significant increase of sensitivity of NMR
  • Development of new in situ and in vivo MRI scanning method.
  • NMR and proteomic research of crystallines of human eye lenses to accumulate new information on formation of cataracts
  • Study of RNA of conjugates with riboflavin and NADH cofactors to achieve understanding of the catalytic role of RNA in formation of life on the Earth
  • Developing high-resolution NMR methods with fast field switching for new experiments in NMR relaxometry and hyperpolarization


The practical value of the study

  • We have synthesized human proteins that are fully tagged with N15 and C13 protein that participate in DNA repair.
  • The Laboratory has conducted research of protein-nucleic interactions in the DNA repair system in a number of synthesized proteins tagged with isotopes С13 and N15 using a high-resolution nuclear magnetic resonance spectrometer.
  • Our researchers have experimentally and theoretically investigated long-lived states whose life span exceeds characteristic relaxation time.
  • We have developed new highly sensitive methods of research of structure and dynamics of biologically important macromolecules.
  • Our researchers has developed methods for creating spin hyperpolarization and transfer of polarization onto selected target spins based on the method of high resolution nuclear magnetic resonance with fast magnetic field switching.
  • Our researchers have developed methods for studying high-rate chemical and biochemical processes with participation of biomacromolecules.
  • We have investigated mechanisms of important catalytic and biocatalytic reactions, detected short-lived intermediates of such processes.
  • We have developed methods of amplification of nuclear magnetic resonance signals by several orders of magnitude for hyperpolarized contrast agents produced using parahydrogen-induced polarization of nuclei in heterogeneous catalysis processes.

Implemented results of research: Cumulative application of tools of magnetic resonance spectroscopy and tomography in accordance with new methods of proteomic analysis has been used: for investigation of post-translational modifications of proteins; to research high-rate reactions with participation of amino acids, nucleotides, peptides ad proteins; for photodynamic and oxidation processes in cataract treatment, as well as for determining molecular mechanisms of industrially important catalysis processes and biocatalysisc reactions.

Education and career development: The Laboratory has developed lecture courses in NMR spectroscopy, including NMR spectroscopy of biomolecules.

Collaborations: Utrecht University (Netherlands): joint scientific publications

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Dobretsov E.A., Snytnikova O.A., Koptyug I.V., Kaptein R., Tsentalovich Y.P.
Magnetic Resonance Imaging (MRI) Study of the Water Content and Transport in Rat Lenses. Experimental Eye Research 113, 162–171 (2013).
Kiryutin A.S., Yurkovskaya A.V., Kaptein R., Vieth H.-M., Ivanov K.L.
Evidence for Coherent Transfer of para-Hydrogen-Induced Polarization at Low Magnetic Fields. Journal of Physical Chemistry Letters 4, 2514–2519 (2013).
Pravdivtsev A.N., Yurkovskaya A.V., Vieth H.-M., Ivanov K.L., Kaptein R.
Level Anti-Crossings are a Key Factor for Understanding para-Hydrogen-Induced Hyperpolarization in SABRE Experiments. CHEMPHYSCHEM 14(14): 3327–3331 (2013).
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