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Invited researcher Alexey Alexeyevich Bogdanov
Time span of the project
2018-2020

As of 22.05.2020

19
Number of staff members
10
scientific publications
General information

Name of the project: Imaging and engineering of eukaryotic genomes


Strategy for Scientific and Technological Development Priority Level: в

Goals and objectives

Project objective: Achieving breakthrough results in mapping images of eukaryotic genomes using multimode tomography and multidisciplinary approaches allowing to assess efficiency of work of the CRISPR/Cas9 systems on different biological models: 2D models of mammal cells, spheroids and 3D models, in small laboratory animals

The practical value of the study

  • We were the first to create lentiviral carrier vectors for doxycycline-regulated expression of chimera proteins based on the catalytically inactive endonuclease Cas9 (dCas9);

  • The Laboratory has conducted experiments in coexpression and localisation in nuclei of chimera protein cells based on dCas9 orthologues;
  • Using a non-cryogenic research device for pre-clinical MRI scanning we have conducted a study of the distribution of mixtures illuminating the surface of skin in subdermal tissue.

Education and career development:

  • We have developed and read a lecture course entitled «Basics of molecular visualization in living systems» for the Faculty of Bioengineering and Bioinformatics of the Moscow State University (Russia).
  • We developed and read a course entitled «Tissue optics and optical clearing of tissues and cells» consisting of three lectures (São Paulo School of Advanced Science on Modern Topics in Biophotonics, Brazil, 2019).
  • We organized the third ADFLIM youth school (advanced methods of fluorescent imaging) for academic training of young researchers and professionals (Saratov, Russia, 2018).
  • We organized the «Saratov Fall Meeting 2018» youth school for optics, laser physics and biophotonics (Saratov, Russia, 2018).
  • Participation in the expert committee for the evaluation of works (project teams) of young researchers in the «Medicine and Pharmacology» section at the 3rd International Scientific Conference «Science of the Future» and the 4th All-Russian Forum «Science of the Future – Science of the Youth».

  • We have conducted the 4th International School on Advanced Fluorescent Imaging Methods on the grounds of the Saratov State University from 23 to 27 September 2019 together with the 23rd International School for Students and Young Scientists on Optics, Laser Physics and Biophotonics. Attendees of the schools also participated in the work of the International Symposium «Optics and Biophotonics - VII» that was held by the Saratov State University and the Federal Research Centre for Biotechnologies of the Russian Academy of Sciences from 24 to 27 September 2019.

  • 6 postgraduates and 2 students have participated in work of the Laboratory.

Other results:

In late May 2019 a state-of-the-art М3™ device for pre-clinical MRI imaging was installed in the laboratory room. The device is used for imaging of the anatomy and physiology of experimental animals (critters weighing up to 30 grams).

The М3™ MRI scanner is built on the basis of a compact highly efficient constant magnet with the magnetic field strength of 1 tesla that does not require cryogenic support. This MRI device is, for now, the only system in Russia and one of about 100 pre-clinical MRI systems installed in leading research centres of the world.

Collaborations:

Laboratory of Laser Diagnostics of Engineering and Living Systems of the Institute of
Precision Mechanics and Control of the Russian Academy of Sciences (Russia),
Laboratory of Biophotonics of the Research and Education Institute for Optics
and Biophootonics of the Saratov State University: joint research and
publications
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Bogdanov A.A., Jr. Solovyev I.D., and Savitsky A.P.
Sensors for Proteolytic Activity Visualization and Their Application in Animal Models of Human Diseases. Biochemistry-Moscow 84(1): 1-18 (2019).
Sdobnov A. Yu., Lademann J., Darvin M. E., Tuchin V. V.
Molecular Optical Imaging Techniques in Dermatology at Skin Optical Clearing. Biochemistry-Moscow 84(1): 144-158 (2019).
Sdobnov A.Yu., Darvin M.E., Schleusener J., Lademann J., Tuchin V.V.
Hydrogen Bound Water Profiles in the Skin Influenced by Optical Clearing Molecular Agents – Quantitative Analysis Using Confocal Raman Microscopy. Journal of Biophotonics e201800283 (2019).
Tuchina D. K., Sindeeva O. A., Savitsky A. P., Bogdanov Jr. A. A., Tuchin V. V.
MRI contrast media as optical clearing agents: perspectives for tissue multimodal imaging. International Conference on Laser Applications in Life Sciences (LALS), November 18-20, 2018, Bar-Ilan University, Israel.
Xie Q., Zeng N., Huang Y., Tuchin V. V., Ma H.
Study on the tissue clearing process using different agents by Mueller matrix microscope. Biomed. Opt. Express.2019. 10(7), 3269–3280.
Leporati A., Gupta S., Bolotin E., Castillo G., Alfaro J., Gottikh M.B., Bogdanov A.A. Jr.
Antiretroviral Hydrophobic Core Graft-Copolymer Nanoparticles: The Effectiveness against Mutant HIV-1 Strains and in Vivo Distribution after Topical Application . Pharm. Res. 2019, 36 (5):73. doi: 10.1007/s11095-019-2604-9.
Munkhbat O., Canakci M., Zheng S., Hu W., Osborne B., Bogdanov A.A., Thayumanavan S.
19F MRI of Polymer Nanogels Aided by Improved Segmental Mobility of Embedded Fluorine Moieties. Biomacromolecules 2019, 20( 2): 790-800. https://doi.org/10.1021/acs.biomac.8b01383
Rodríguez-Rodríguez A., Shuvaev S., Rotile N., Jones C.M., Probst C.K., Ferreira D.D.S., Graham-O′Regan K., Boros E., Knipe R.S., Griffith J.W., Tager A.M., Bogdanov Jr. A.A., Caravan P.
Peroxidase Sensitive Amplifiable Probe for Molecular Magnetic Resonance Imaging of Pulmonary Inflammation. ACS Sens. 2019, 4(9): 2412-2419, https://doi.org/10.1021/acssensors.9b01010
Carneiro I, Carvalho S, Henrique R, Oliveira L, Tuchin V.
Moving tissue spectral window to the deep-ultraviolet via optical clearing. J. Biophotonics. 2019, 12 (12), e201900181. https:// doi.org/10.1002/jbio.201900181.
Taghian, T, Metelev, VG, Zhang, S, Bogdanov, AA Jr.
Imaging NF-κB activity in a murine model of early stage diabetes. The FASEB Journal 2020, 34: 1198–1210.
Metelev VG, Bogdanov AA Jr
Synthesis and applications of theranostic oligonucleotides carrying multiple fluorine atoms. Theranostics 2020, 10 (3), 1391.
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