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Invited researcher Paul Schedl
Contract number
14.B25.31.0022
Time span of the project
2013-2017
Head of the laboratory

As of 15.02.2021

15
Number of staff members
47
scientific publications
General information

Name of the project: Study of molecular basics gene expression regulation in development of Drosophila

Strategy for Scientific and Technological Development Priority Level: в


Goals and objectives

Research directions: At the Laboratory we research molecular basics of regulation of functioning of genes in the process of organism development

Project objective: At the Laboratory we research molecular basics of regulation of functioning of genes in the process of organism development


The practical value of the study

  • We have researched the role of proteins of the CPEB family – orb and orb2 – in regulation of polarization of tissues and development of testes and ovaries in drosophila. We have also researched mechanisms of control of specification and status support of status of primary stem cells in gonads of drosophila.
  • Using a model of the bithorax complex we has investigated new basic factors that support remote interactions in drosophila embryos. A comparative description of various insulators of the bithorax gene complex has been presented.
  • We have researched mechanics of action of Elba, Insv, ENY2, SAYP, LBC proteins and protein complexes in regulation of activity of genes during development of Drosophila.
  • Our researchers have studied molecular basics of dosage compensation in drosophila, mechanisms of recruitment of dosage compensation complexes onto the X chromosome in Drosophila.
  • The mechanisms controlling the activity of enhancers, the functioning of insulators, and the formation of higher chromatin structures have been investigated. The structure of architectural factors of chromatin, transcription factors, and RNA-binding proteins that control gene expression have been studied. New models of various stages of the gene expression process have been proposed. The molecular basis of a number of processes of ontogenesis, the formation of cell polarity, and the functioning of the nervous system have been revealed.

Education and career development:

  • More than 10 graduate projects of masters and specialists, 7 PhD and 1 DSc theses have been completed and defended.

  • A school-conference for young scientists was held.

  • Students of the Russian National Research Medical University named after N.I. Pirogov are being trained.

Organizational and structural changes:

Laboratory participates in the work of the Center for Precision Genome Editing and Genetic Technologies for Biomedicine of the IBG RAS

Collaborations:

  • Princeton University (USA), joint research, publications

  • The Scripps Research Institute, USA, joint research, publications

  • Università degli Studi di Napoli "Federico II", Italy, joint research, publications

  • Institute of research on terrestrial ecosystems (IRET), CNR, Italy, joint research, publications

  • FAME Laboratory, Centre for Research and Technology Thessaly, Greece, joint research, publications

  • Laboratory of Immunopathology, Instytut Immunologii i Terapii Doświadczalnej im. Ludwika Hirszfelda Polskiej Akademii Nauk, Poland, joint research, publications

  • Scientific Center for Genetics and Life Sciences, Sirius University, Sochi, joint research, publications

  • Laboratory of Intracellular Signal Transmission in Norm and Pathology, Institute of Molecular Biology, Russian Academy of Sciences, Moscow, joint research, publications

  • Laboratory of Biochemical Genetics of Animals, Institute of Molecular Genetics, Moscow, joint research, publications

  • Laboratory of Neurogenetics, Institute of Physiology. I.P. Pavlova RAS, St. Petersburg, joint research, publications

  • Scientific and educational center "Photonics and infrared technology" Moscow State Technical University named after N.E. Bauman, Moscow, joint research, publications

  • Department of Biology and General Genetics, Faculty of Medicine, Sechenov University, Moscow, joint research, publications

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Maksimenko O, Bartkuhn M, Stakhov V, Herold M, Zolotarev N, Jox T, Buxa MK, Kirsch R, Bonchuk A, Fedotova A, Kyrchanova O, Renkawitz R, Georgiev P.
Two new insulator proteins, Pita and ZIPIC, target CP190 to chromatin. Genome Res. (2015) 25(1):89-99.
Erokhin M., Elizar'ev P., Parshikov A., Schedl P., Georgiev P., Chetverina D.
Transcriptional read-through is not sufficient to induce an epigenetic switch in the silencing activity of Polycomb response elements. Proc Natl Acad Sci USA. (2015) 112(48): 14930–14935
Kyrchanova O., Mogila V., Wolle D., Deshpande G., Parshikov A., Cléard F., Karch F., Schedl P., Georgiev P.
Functional Dissection of the Blocking and Bypass Activities of the Fab-8 Boundary in the Drosophila Bithorax Complex. PLoS Genetics (2016) 12(7): e1006188.
M. Fujioka, H. Mistry, P. Schedl, J. Jaynes
Determinants of Chromosome Architecture: Insulator Pairing in cis and in trans. PLoS Genet (2016) 24;12(2):e1005889.
Postika N, Metzler M, Affolter M, Müller M, Schedl P, Georgiev P, Kyrchanova O
Boundaries mediate long-distance interactions between enhancers and promoters in the Drosophila Bithorax complex. PLoS Genet. (2018) 14(12):e1007702.
Kyrchanova O, Kurbidaeva A, Sabirov M, Postika N, Wolle D, Aoki T, Maksimenko O, Mogila V, Schedl P, Georgiev P.
The bithorax complex iab-7 Polycomb response element has a novel role in the functioning of the Fab-7 chromatin boundary. PLoS Genet. (2018) 14(8):e1007442.
Barr J., Charania S., Gilmutdinov R., Yakovlev K., Shidlovskii Y., Schedl P.
The CPEB translational regulator, Orb, functions together with Par proteins to polarize the Drosophila oocyte. PLoS Genetics(2019) 15(3):e1008012
Kyrchanova O, Sabirov M, Mogila V, Kurbidaeva A, Postika N, Maksimenko O, Schedl P, Georgiev P.
Complete reconstitution of bypass and blocking functions in a minimal artificial Fab-7 insulator from Drosophila bithorax complex. Proc Natl Acad Sci USA. (2019); 116(27):13462-13467.
Kyrchanova O, Maksimenko O, Ibragimov A, Sokolov V, Postika N, Lukyanova M, Schedl P, Georgiev P.
The insulator functions of the Drosophila polydactyl C2H2 zinc finger protein CTCF: Necessity versus sufficiency. Sci Adv. (2020) 6(13):eaaz3152.
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