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Laboratory of Expression Systems and Plant Genome Modification (BIOTRON)

Invited researcher Alexander Mikhailovich Vainstein
Contract number
14.B25.31.0027
Time span of the project
2013-2015
Laboratory's website
Head of the laboratory
Sergey Vladimirovich Dolgov

As of 30.01.2020

33
Number of staff members
1
Objects of intellectual property
General information

Name of the project: Development of expression systems based on transgenic agricultural plants for producing substances for veterinary and medical applications.

Strategy for Scientific and Technological Development Priority Level: г


Goals and objectives
Research directions: Plant genetic engineering, development of agrobacterial and ballistic methods of production of transgenic agrobacterial fruit, berry, vegetable and decorative cultures with new economically important properties

Project objective: Development of expression systems based on transgenic agricultural plants for producing substances for veterinary and medical applications, including development of a strategy of production of edible vaccines against rabies for agricultural animals; growing plants – producers of «universal» edible vaccines against bird and swine flu; production of plants that produce artemisin, an anti-malaria agent



The practical value of the study

  • We have produced vector structures for synthesis of synthesis of vector proteins against flu, rabies as well as the artemisin anti-malaria agent in plant.
  • We have produced first transgenic plants of common duckweed, tobacco, wolffia arrhiza and chrysantheme that express these genes.
  • Our researchers have studied dynamics of growth of duckweeds with content of crude protein and dry matter in plants depending on composition of cultivation environments. As a result of research we selected a composition of environment that is optimal for cultivation of duckweeds – the optimal concentration of macro- and microelements and sucrose.
  • The Laboratory has developed protocols of extraction and partial purification of vaccine proteins containing a ricin B subunit. We have developed buffers that are optimal for extraction of proteins from tobacco and duckweed plant biomass; a protocol has been developed for deep purification of recombinant proteins from admixtures and their concentration using differential fractionation by ammonium sulfate.

Collaborations: Nikitsky Botanical Gardens (Russia), Main Botanical Garden of the Russian Academy of Sciences (Russia), All-Russian Research Institute of Agricultural Biotechnology (Russia), Southern Federal University (Russia): collaborative research

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Firsov A., Tarasenko I., Mitiouchkina T., Ismailova N., Shaloiko L., Vainstein A., Dolgov S.
High-Yield Expression of M2e Peptide of Avian Influenza Virus H5N1 in Transgenic Duckweed Plants. Molecular Biotechnology 57(7): 653–661 (2015).
Firsov A.P., Tarasenko I.V., Pushin A.S., Shaloiko L.A., Vinokurov L.M., Dolgov S.V.
Expression in Tobacco Transgenic Plants of a Gene for M2e Peptide of H5N1 Avian Influenza Virus in Translational Fusion with Ricin B Subunit // Biotechnology in Russia. 2015. № 2. С. 55–64.
Khvatkov P., Chernobrovkina M., Okuneva A., Pushin A., Dolgov S.
Transformation of Wolffia arrhiza (L.) Horkel ex Wimm. Plant Cell, Tissue and Organ Culture (PCTOC) 123(2): 299–307 (2015).
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