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Invited researcher Leonid Leonidovich Moroz
Contract number
14.W03.31.0015
Time span of the project
2017-2019
Head of the laboratory

As of 15.02.2021

5
Number of staff members
17
scientific publications
1
Objects of intellectual property
General information

Name of the project: Reconstruction of genealogy of cell systems based on analysis of biodiversity of the World Ocean

Strategy for Scientific and Technological Development Priority Level: а, в


Goals and objectives

Research directions: High performance sequencing and analysis of genomes/transcriptomes at the level of single cells (single-cell-Seq), molecular deciphering of the global biodiversity of marine organisms and molecular and genetic analysis of nervous systems at the level of single cells, identification and analysis of cell types based on results of high performance sequencing of transcriptomes of single cells

Project objective: Development of approaches and methods for reconstruction of genealogy of neurons and their ancestor lines on the basis of research of genomics of marine organisms at the level of separate cells; development of criteria of natural classification of neurons in neuron networks for thous types of marine animals that have key importance for understanding of evolution and for biomedicine


The practical value of the study

We have refined phylogeny of animals and developed methods of quantitative assessment of transcription activity of genome in cells of tested species. More than 1.5 million transcriptomes of single neurons were obtained in representatives of 16 phyla of animals.

Our researchers have shown that in the majority of researched species almost all neurons have unique profile of non-coding RNA and secretory molecules which creates the basis for development of natural evolutionary classification of neurons. We have found vast molecular diversity of «simple» nervous systems.

We have confirmed that formation of neuronal phenotypes from various types of ancestor secretory cells could occur at least twice or three times during the evolution of animals. Muscle cells could evolve independently.

Reconstruction of evolution of centralization of the nervous system allows to suggest that formation a complex brain went independently at least 9–12 times from the common ancestor Bilateria and Cnidaria with diffuse nervous system.

Education and career development:

Based on the Science education centre "Genomics and Bioinformatics" some programs of professional training and popularization of the scientific knowledge in the field of marine biology and functional genomics are implemented. Practical courses are held using modern laboratory equipment. Several students from Sevastopol State University have realized magisters works based on the laboratory as well three post-graduate students are carrying out research nowadays. Lectures and practical courses conducted in the framework of the training programs for SevSU Master's degree programs in "biophysics".

Collaborations:

Institute of Cytology and Genetics of the Siberian Department of the Russian Academy of Science (Russia): Joint research, internships for laboratory staff, scientific conferences

Sevastopol State University (Russia): Training of personnel in the field of bioinformatics analysis, the use of the instrumental base of the Laboratory of Molecular and Cellular Biophysics for experimental molecular work.

Nikitsky Botanical Garden (Russia): coordinating development plans for cutting-edge research directions

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N.V. Whelan, K. M. Kocot, T. P. Moroz, K. Mukherjee, P. Williams, G. Paulay, L.L. Moroz, K.M. Halanych
Ctenophore relationships and their placement as the sister group to all other animals // Nature Ecology & Evolution. - VOL 1, NOVEMBER 2017. Р. 1737–1746.
Naumenko F., Abnizova I., Beka N., Orlov Y.
Novel read density distribution score shows possible aligner artefacts, when mapping a single chromosome BMC Genomics V18, Suppl 11, 2017
Norekian TP, Moroz LL.
Comparative neuroanatomy of ctenophores: Neural and muscular systems in Euplokamis dunlapae and related species. J Comp Neurol. 2019 September 9. DOI: 10.1002/cne.24770
Zverkov OA, Mikhailov KV, Isaev SV, Rusin LY, Popova OV, Logacheva MD, Penin AA, Moroz LL, Panchin YV, Lyubetsky VA, Aleoshin VV.
Dicyemida and Orthonectida: Two Stories of Body Plan Simplification //. Front Genet. 2019 May 24;10:443. doi: 10.3389/fgene.2019.00443. eCollection 2019. PMID: 31178892
E.A. Vodiasova , E.S. Chelebieva, O.N. Kuleshova
The new technologies of high-throughput single-cell RNA sequencing // Vavilov’s Journal of Genetics. - 2019;23(5):508-518 https://doi.org/10.18699/VJ19.520
Gubanova A., Drapun I., Garbazey O., Krivenko O., Vodiasova E.
Pseudodiaptomus marinus Sato, 1913 in the Black Sea: morphology, genetic analysis, and variability in seasonal and interannual abundance // PeerJ. 2020. Iss. 8. Articleno. e10153 (26 p.). DOI: 10.7717/peerj.10153
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