[EN] Лаборатории

Invited researcher Yuriy Vasilyevich Kotelevtsev

Contract number

11.G34.31.0032

Time span of the project

2010-2012

As of 30.01.2020

Number of staff members

scientific publications

Name of the project: Targeted stem cell genome modification as a platform for searching for new pharmaceuticals

Strategy for Scientific and Technological Development Priority Level: в

Research directions: Producing new knockout mouse lines immune to atherosclerosis, methods of stem cell genome modification and in vivo investigation of processes occurring in vessel walls, methods of of two-photon laser scanning microscopy; creating «cosmetics» containing hyaluronan for vessels

Project objective:

Research of possible ways for correction of atherosclerosis
Search for new targets for medications using targeted genome modification

We have created a bank of E.Coli strains and plasma vectors carrying genetic structures necessary for targeted genome modification.
Our researchers have prepared lines of the 129Ola embryo stem cells and tested methods of their modification and cloning.
Optical methods for research of glycocalyx. Data has been obtained on the key role of vessel endothelium glycocalyx in development of atherosclerosis.
We have refined the method of implanting telemetric sensors for ECG.
We have refined the method of hypertrophy of vessel walls in response to mechanical damage of endothelium.
We have created in vitro and in vivo models that allow us to analyze drugs and search for new targets for treatment of the Alzheimer's disease.
Our researchers have refined a method of immunoferment detection of syndekane in mouse blood plasma. A comparison of a commercial set for determining human syndekane with antibodies to syndekane-1 of mice produced at our Laboratory. We have shown high specificity of the method that allows to determine amount of syndekane diluted in plasma. We have also shown that in the process of development of atherosclerosis in mice of the ApoE line concentration of syndekane increases. We have drawn blood samples from mice with different stages of atheroma development.

Education and career development: 6 masters dissertations have been defended

Collaborations:

University of Edinburgh (United Kingdom), A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences (Russia): joint research

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Laman A.G., Lathe R., Savinov G.V., Shepelyakovskaya A.O., Boziev Kh.M., Baidakova L.K., Chulin A.N., Brovko F.A., Svirshchevskaya E.V., Kotelevtsev Y., Eliseeva I.A., Guryanov S.G., Lyabin D.N., Ovchinnikov L.P., Ivanov V.T.

Innate immunity: Bacterial cell-wall muramyl peptide targets the conserved transcription factor YB-1. FEBS Letters 589(15): 1819-24 (2015).

Kirkby N.S., Duthie K.M., Miller E., Kotelevtsev Y.V., Bagnall A.J., Webb D.J. and Hadoke P. W.F.

Non-endothelial Cell Endothelin-B Receptors Limit Neointima Formation Following Vascular Injury. Cardiovascular Research 95(1): 19–28 (2012).

Deuchar G.A., Patrick M.D., Hadoke W.F., Brownstein D.G., Webb D.J., Mullins J.J., K. Chapman, Seckl J. R. and Kotelevtsev Y.V.

11-Hydroxysteroid Dehydrogenase Type 2 Deficiency Accelerates Atherogenesis and Causes Proinflammatory Changes in the Endothelium in Apoe / Mice. Endocrinology 152(1): 236–246 (2011).

Hosting organization	Field of studies	City	Invited researcher	Time span of the project
Laboratory of Super-elastic Bio-interfaces National Research Tomsk State University	Medical biotechnologies	Tomsk	Volinsky Alexei USA	2021-2023
Laboratory of Clinical Smart Nano-technologies I.M.Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation (Sechenovskiy University)	Medical biotechnologies	Moscow	Liang Xing-Ji China	2021-2023
Laboratory of Laser Molecular Imaging and Machine Learning National Research Tomsk State University	Medical biotechnologies	Tomsk	Igor Konstantinovich Lednev Russia	2021-2023

Laboratory for Stem Cell Genome Modification