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Invited researcher Chikindas Michael Leonidas
Contract number
075-15-2019-1880
Time span of the project
2019-2021

As of 15.02.2021

53
Number of staff members
10
scientific publications
8
Objects of intellectual property
General information

Name of the project: Veterinary probiotic medications for targeted modulation of health of animals

Strategy for Scientific and Technological Development Priority Level: г

Goals and objectives

Project objective: Development of systems of key «algorithms» aimed at creation of new technologies for production of efficient probiotic medications for applications in veterinary optimized for animal organisms.

The practical value of the study

We have researched the biota of the gastrointestinal tract (GIT) in meat and egg laying chickens in the context of various breeding technologies. It has been determined that the GIT micro-biota is mainly affected by the ration of the birds. A primary collection of potential probiotic micro-organisms has been formed. It consists of 19 strains of sporulating bacilli and 36 strains of lactic acid bacteria. These micro-organisms have been tested for anti-oxidation and DNA protection activity. On the basis of the obtained results, we have isolated ten strains that are the most promising for further use and deposited them to the All-Russian National Collection of Industrial Micro-organisms (VKPM): the bacilli KB16, KB18, KB41, KB44, KB54 and the lactic acid bacteria KL31, KL61, KL62, KL73, KL82.

Our researchers have developed a software & hardware system that is equivalent to «SHIME – The Simulator of the Human Intestinal Microbial Ecosystem» artificial colon model. The system can be used to research processes in the gastrointestinal tracts of domestic poultry. It ensures the staging of micro-biological experiments in an environment with managed and controlled parameters. A hierarchical micro-processor-based control and monitoring system has been developed that ensures the conduction of experiments of arbitrary duration with a controlled chyme temperature and рН while maintaining an inert atmosphere, The reactors of the models can be scaled and linked into chains operating with a common automated technological process control system. The complex ensures the recording of the conditions of the conduct of experiments in terms of the controlled parameters. The purchase of related equipment allowed to organise several laboratories that form a common infrastructure for the work of artificial colon models.

The strains KB16, KB18, KB41, KB44, KB54 as well as Bacillus subtilis KATMIRA1933 have been tested for survivability and the secretion of lytic enzymes, anti-oxidants, and DNA-protective substances in the context of an artificial chicken colon. For this purpose, a new artificial colon environment has been developed that imitates the chicken colon in terms of its content. It has been demonstrated that in the conditions of the GIT, the secretion of lytic enzymes by bacilli is reduced in the same manner as the secretion of anti-oxidative and DNA-protective compounds. The bacillus strains start escaping spores as early as in the crops of birds, which reduces their survivability in the stomach. However, the strains KB16, KB41, KB54 are able to colonise the colons of birds if they pass the gastric acid.

Within the created Center for Agrobiotechnology of the Don State Technical University, an experimental rig has been created, using which we have developed a laboratory technology for the production of probiotic agents, conditions have been selected for solid-phase cultivation providing the most active agents, pilot batches of probiotic agents have been accumulated for research. Work has been conducted to identify metabolites of probiotic bacilli by means of chromatography, Our researchers have also isolated the active fractions of metabolites. A method has been proposed for the production of inembryonate biological preparations based on metabolites of probiotic bacilli and fodders based on them.

Implemented results of research:

The Laboratory has developed a technology for the production of a bio-preparation with anti-fungal activity on the basis of solid-phase fermentation of spore-forming bacilli on a plant-based substrate, the area of application is agro-bio-technology (plant breeding). The preparation was produced on the grounds of the laboratory «‎Centre for agro-bio-technology» for an industrial customer.

Education and career development:

  • An academic team has been formed that includes young scientists, postgraduates and students of the Don State Technical University. In 2020-2021, 6 certificates of completion of additional training were obtained by our staff members.
  • A memorandum of collaboration has been signed between Maastricht University (the Netherlands) and the Don State Technical University. In 2021, we are planning 30-day academic internships at Maastricht University for four employees of the Laboratory.
  • We are providing training to professionals of highest qualification (7 Candidates of Science and one Doctor of Science).
  • The results of the development of the model of an artificial GIT of chickens are used to conduct training in the following courses: «Modelling of control systems» (degree programme «Control of engineering systems»), «Modelling of systems and processes» (degree programme «Automation of technological processes and manufacturing lines»), «SCADA» (degree programme «Industrial programming»). The results of our work are also used in two postgraduate degree programmes.
  • We have developed and are implementing the additional training programme «Innovative approaches in the creation of veterinary probiotic preparation for the targeted modulation of animal health».
  • The main education programme «Game management, cynology, and zookeeping» within the degree programme «Zootechnics» has been developed and is planned for introduction.
  • The Laboratory has developed and is currently implementing main education programmes «Processes and equipment in bio-technology» for the degree programme «Processes and equipment in bio-technology», «Genetics and breeding of fish» for the degree programme «Aquatic bio-resources and aquaculture», «Fish farming» for the degree programme «Aquatic bio-resources and aquaculture».
  • The programmes and equipment developed within the project are currently being adapted for the use as a base for case studies and term projects in the specified subjects, methodological materials are being developed for them.
  • We are developing the experimental and training field of the Don State Technical University, creating grounds for the implementation of scientific and technological ideas in the domain of fodder production, including fodder with the addition of probiotics.

Collaborations:

  • Amazone Werke, Wintersteiger AG, centre for engineering solutions in aquiculture «Simeon AquaBioTechnologies», Lemken GmbH & Co. KG, «Bizon» agriculture technology holding, «Rostselmash» harvester plant, «Bonum» machinery plant, Research Institute of Agriculture of Crimea, agriculture research centre «Donskoy»: joint scientific research.
  • Russian Academy of Sciences, Southern Scientific Centre of the Russian Academy of Sciences, Rutgers University (USA, «Bonum» machinery plant, Lallemand Animal Nutrition, AGROCOM GROUP LLC, Amazone Werke, Wintersteiger AG, «Bizon» agriculture technology holding, «Rostselmash» harvester plant, Lemken GmbH & Co. KG: joint conferences and forums: the international science and practice conference «The state and the prospects of the development of agro-industrial complex», the international science and practice conference «Mathematical modelling and bio-mechanics», the international conference «ICOBM-2021», the international science and practice conference «Innovative technologies in science and education», the international science and practice conference «The development and modern problems of aquiculture», the international science and practice conference «Modern tendencies in machine engineering and technosphere safety», the international forum «The youth in the agro-industrial complex», the international science and practice conference «Innovative achievements of world science in agricultural production».
  • Rutgers University (USA), South China Agricultural University (China PR), Zhejiang University (China PR), Shanghai Jiao Tong University (China PR), University of Agriculture Faisalabad (Pakistan), University of Diyala (Iraq), Maharshi Dayanand University (India), Stellenbosch University (South Africa): collaborative publications, joint presentations at international conferences.
  • Russian Academy of Sciences, Southern Federal University, Rostov State Medical University, National Medicine Research Centre of Oncology, Institute of Experimental Medicine, Saint Petersburg State University, I. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Russia): publication of works by unified teams in highly ranked academic journals, preparation of joint keynotes and delivery of presentations at international conferences as well as the development of education activities.

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Mahoney, R., Weeks, R., Huang, Q., Dai, W., Cao, Y., Liu, G., Guo, Y., Chistyakov, V. A., Ermakov, A. M., Rudoy, D., Bren, A., Popov, I., & Chikindas, M. L.
Duckweed as a Potential Feed Additive with Poultry Beneficial Bacilli Probiotics [published online ahead of print, 2021 May 14]. Probiotics Antimicrob Proteins. 2021;10.1007/s12602-021-09794-4. doi:10.1007/s12602-021-09794-4
Abdullah, A. Asghar, A. Algburi, Q. Huang, T. Ahmad, H. Zhong, H.U. Javed, A.M. Ermakov, and M.L. Chikindas
Anti-biofilm potential of Elletaria cardamomum (green cardamon) essential oil against Escherichia coli O157:H7 and Salmonella Typhimurium JSG 1748. Frontiers in Microbiology 2021. April, v. 12:620227. doi: 10.3389/fmicb.2021.620227
K.A. Demin, A.G. Refeld, A.A. Bogdanova, E.V. Prazdnova, I.V. Popov, O. Ju. Kutsevalova, A.M. Ermakov, A.B. Bren, D.V. Rudoy, V.A. Chistyakov, and M.L. Chikindas
Mechanisms of Candida Resistance to Antimycotics and Promising Ways to Overcome It: The Role of Probiotics [published online ahead of print, 2021 Mar 18]. Probiotics Antimicrob Proteins. 2021;10.1007/s12602-021-09776-6. doi:10.1007/s12602-021-09776-6
Donnik, I.M., Popov, Ig.V., Sereda, S.V., Popov, Il.V., Chikindas,M.L., and A. M. Ermakov. Donnik IM, Popov IV, Sereda SV, Popov IV, Chikindas ML, Ermakov AM.
Coronavirus Infections of Animals: Future Risks to Humans. Biol Bull Russ Acad Sci. 2021;48(1):26-37. doi:10.1134/S1062359021010052
M. Hassanpour Tazehabadi, A. Algburi, I.V. Popov, A.M. Ermakov, V.A. Chistyakov, E. Prazdnova, R. Week, and M.L. Chikindas
Probiotic bacilli inhibit Salmonella biofilm formation without killing planktonic cells. Frontiers in Microbiology 2021. April, v. 12:615328. doi: 10.3389/fmicb.2021.615328
Kumar, S. Tewari, J. Tagg, M.L. Chikindas, I. Popov, and S.K. Tiwari
Can probiotics emerge as effective therapeutic agents in apical periodontitis? A Review. Probiotics Antimicrob Proteins. 2020. v. 13: 299–314. doi: 10.1007/s12602-021-09750-2. [Epub ahead of print]
S.K. Tiwari, L.M.T. Dicks, I.V. Popov, A. Karaseva, A.M. Ermakov, A. Suvorov, J.R. Tagg, R. Weeks and M.L. Chikindas
Probiotics at war against viruses: What is missing from the picture? Frontiers in Microbiology 2020. v. 11:1877. doi: 10.3389/fmicb.2020.01877
Algburi, H.M. Al-Hasani, T.K. Ismael, A. Abdelhameed, R. Weeks, A.M. Ermakov, M.L. Chikindas
Antimicrobial Activity of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 Against Staphylococcus aureus Biofilms Isolated from Wound Infection. Probiotics Antimicrob Proteins. 2021;13(1):125-134. doi:10.1007/s12602-020-09673-4
Algburi, S.A. Alazzawi, A.I. Ali Al-Ezzy, R. Weeks, V. Chistyakov, and M.L. Chikindas
Potential Probiotics Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 Co-Aggregate with Clinical Isolates of Proteus mirabilis and Prevent Biofilm Formation. Probiotics Antimicrob Proteins. 2020;12(4):1471-1483. doi:10.1007/s12602-020-09631-0
S. Sushkova, T. Minkina, V. Chaplygin, D. Nevidomskaya, V. Rajput, T. Bauer, M. Mazarji, A.B. Bren, I. Popov, M. Mazanko
Subcritical water extraction of organic acids from chicken manure. J Sci Food Agric. 2021;101(4):1523-1529. doi:10.1002/jsfa.10768
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