We use cookies.
By using the site, you agree to our Privacy Policy.

Contract number
11.G34.31.0015
Time span of the project
2010-2012

As of 30.01.2020

17
Number of staff members
17
scientific publications
General information

Name of the project: Nanostructuring of membrane-protein complexes for controlling cell physiology

Strategy for Scientific and Technological Development Priority Level: в


Goals and objectives

Research directions: Experimental research of fundamental mechanisms of formation of specific sources of tachyarrhythmia, computer modeling of processes of propagation of disturbance in heart tissue, development of methods of photocontrol of heart tissue, research of the fundamental mechanism of formation of arrhythmia in people suffering from manifestations of various mutations leading to functional cardiac changes.

Project objective:

  • Creation of artificial cardiac tissue patches suitable for future implantation and replacement of damaged regions of the heart
  • Research of pathological processes leading to formation of dangerous cardiac arrhythmia such as rotating wave disturbance («reentry») causing disorganization of the heart muscle with loss of ability to support blood circulation
  • Creation of an implantable patch that will be fully immune-compatible with organisms of donor-recipient


The practical value of the study

  • Our researchers have solved the problem of unpinning within the research of mechanisms of formation of specific sources of tachyarrhythmia. We have found out that cell orientation and organization in heart tissue play quite an important role in conduction. It has been determined that ectopic sources can occur on boundaries of domains.
  • The Laboratory has conducted analysis of various matrices and their forms for further usage as transplantation materials or for modeling. We have acquired brand new information concerning formation of cells on tissue.
  • We have developed a unique cell model of the cardiac monolayer. Using computer modeling we have demonstrated effects occurring as a result of various degrees of anisotropy in cardiac tissue.
  • In the domain of photocontrol we have conducted research of toxicity and properties of a group of azobenzenes (АзоТаб) as well as performed works in coalescence of cardiac structures of various types and their stimulation using light.

Implemented results of research:

  • We have produced the photocontrolling material called AsoTab using which it is possible to perform temporary ablation. Its possible applications are prevention and treatment of arrhythmia in cardiac surgery.
  • The computer model completely forecasting various behaviors of disruption wave in a culture of cardiomyocytes that accounts for formation of heart tissue can be used for forecasting and predicting of arrhythmia in cardiac disease medicine.
  • The test system for studying formation of arrhythmia under impact of various medication can be used in the pharmaceuticals industry for developing and testing new medications.

Education and career development:

  • 4 candidate dissertations have been defended

Other results: On the basis of human cardiomyocytes extracted from induced pluripotent stem cells we have created a cell engineering model that allows to conduct research in cardiotoxicity and efficiency of antiarrhythmic medications (results have been published in the official journal of the Society of Toxicology).

Hide Show full
Kudryashova N., Tsvelaya V., Agladze K., Panfilov A.V.
Virtual cardiac monolayers for electrical wave propagation. Scientific Reports 7(1): 7887 (2017).
Balashov V., Efimov A., Agapova O., Pogorelov A., Agapov I., Agladze K.
High Resolution 3D Microscopy Study of Cardiomyocytes on Polymer Scaffold Nanofibers Reveals Formation of Unusual Sheathed Structure. Acta Biomaterialia 68: 214–222 (2017).
Kudryashova N.N., Kazbanov I.V., Panfilov A.V., Agladze K.I.
Conditions for Waveblock Due to Anisotropy in a Model of Human Ventricular Tissue. PLoS ONE 10(11): e0141832 (2015).
Frolova S.R, Gaiko O., Tsvelaya V.A., Pimenov O.Y., Agladze K.I.
Photocontrol of Voltage-Gated Ion Channel Activity by Azobenzene Trimethylammonium Bromide in Neonatal Rat Cardiomyocytes. PLoS ONE 11(3): e0152018 (2016).
Slotvitsky M., Tsvelaya V., Frolova S., Dementyeva E., Agladze K.
Arrhythmogenicity Test Based on a Human-Induced Pluripotent Stem Cell (iPSC)-Derived Cardiomyocyte Layer. Toxicological Sciences 168(1): 70-77 (2019).
Other laboratories and scientists
Hosting organization
Field of studies
City
Invited researcher
Time span of the project
Laboratory of Super-elastic Bio-interfaces

National Research Tomsk State University

Medical biotechnologies

Tomsk

Volinsky Alexei

USA

2021-2023

Laboratory of Clinical Smart Nano-technologies

I.M.Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation (Sechenovskiy University)

Medical biotechnologies

Moscow

Liang Xing-Ji

China

2021-2023

Laboratory of Laser Molecular Imaging and Machine Learning

National Research Tomsk State University

Medical biotechnologies

Tomsk

Igor Konstantinovich Lednev

Russia

2021-2023