МЕГАГРАНТЫ

Лаборатория молекулярной микробиологии

О лаборатории

Наименование проекта Молекулярная, экологическая и прикладная микробиология

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№ договора:
14.B25.31.0004

Наименование ВУЗа:
ФГБОУ ВПО "Санкт-Петербургский государственный политехнический университет"

Области научных исследований:
Промышленные биотехнологии


ЦЕЛЬ ПРОЕКТА
Создание в СПбГПУ современной лаборатории молекулярной, экологической и прикладной микробиологии.
ЗАДАЧИ ПРОЕКТА
— Исследования молекулярных механизмов транскрипции прокариот и способов ее регуляции. В частности, выявление из природных источников новых бактериофагов, заражающих бактерии, их геномная и функциональная характеристика, идентификация, функциональная и структурная характеристика транскрипционных факторов фагов и потенциально полезных фаговых белков.
— Разработка методов анализа на уровне одной клетки для изучения и, в конечном счете, контроля стохастических процессов в бактериальных клетках, с использованием в качестве экспериментальной модели заражения бактериофагом клеток, несущих системы рестрикции-модификации и системы адаптивного иммунитета CRISPR/Cas.

Ведущий учёный

severinov 

ФИО: Северинов Константин Викторович

 

Ученые степень и звание:
Доктор биологических наук, профессор

Занимаемая должность:
Профессор университета Ратгерс, Нью Брунсвик, США, профессор Сколтех, зав. лабораториями в ИМГ РАН и ИБГ РАН

Области научных интересов:

Молекулярные основы регуляции экспрессии генов прокариот, молекулярные основы и эволюция взаимодействий бактерий и их вирусов, структура и механизм действия новых антибиотиков

Научное признание:

- Fulbright Senior Scholar, 2010;
- Fellow of American Academy of Microbiology, 2009;
- The Russian Science Support Foundation Prize, 2008, 2009;
- National Research Council Exchange Fellowship with All-Russian State Center for Applied Microbiology, Obolensk, Russia, 2004;
- Participant, National Research Council Exchange Program with former Russian Bioweapons Institutes, 2002;
- 10th Johnson & Johnson Discovery Award, 1997;
- The Burroughs Wellcome Fund Career Award in the Biomedical Sciences, 1996 – 2002;
- The Jane Coffin Childs Memorial Fund for Medical Research postdoctoral fellowship, 1994 – 1997;
- The Soros Foundation-USSR studentship, 1991;
- The British Council scholarship, 1990.


1. Severinov, K., Melnikova, E., and Ryazanov, A. (1990) Down-regulation of the translation elongation factor 2 kinase in Xenopus laevis oocytes at the final stages of oogenesis. New Biol., 2, 887-893
2. Price, N., Redpath, N., Severinov, K., Campbell, D., Russel, R., and Proud, C. (1991) Identification of the phosphorylation sites in elongation factor 2 from rabbit reticulocytes. FEBS Lets., 282, 253-258
3. Borukhov, S., Severinov, K., Kashlev, M., Lebedev, A., Bass, I., Rowland, G. C., Lim, P-P., Glass, R. E., Nikiforov, V., and Goldfarb, A. (1991) Mapping of trypsin cleavage and antibody binding sites and delineation of a dispensable region in the β subunit of Escherichia coli RNA polymerase. J. Biol. Chem., 266, 23921-23926
4. Severinov, K., Mustaev, A., Kashlev, M., Borukhov, S., Nikiforov, V., and Goldfarb, A. (1992) Dissection of the β subunit in the E. coli RNA polymerase into domains by proteolitic cleavage. J. Biol. Chem., 267, 12813-12819
5. Redpath, N., Price, N., Severinov, K., and Proud, C. (1993) Regulation of elongation factor 2 by multisite phosphorylation. Eur. J. Biochem., 213, 689-699
6. Severinov, K., Soushko, M., Goldfarb, A., and Nikiforov, V. (1993) Rifampicin region revisited: New Rifampicin-resistant and Streptolydigin-resistant mutants in the β subunit of Escherichia coli RNA polymerase. J. Biol. Chem., 268, 14280-14825
7. Kashlev, M., Martin, E., Polyakov, A., Severinov, K., Nikiforov, V., and Goldfarb, A. (1993) Histidine-tagged RNA polymerase: Dissection of the transcription cycle using immobilized enzyme. Gene, 130, 9-14
8. Ross, W., Gosink, K. K., Salomon, J., Igarashi, K., Zou, K., Ishihama, A., Severinov, K., and Gourse, R. L. (1993) A third recognition element in prokaryotic promoters: Site-specific DNA binding by the α subunit of RNA polymerase. Science, 262, 1407-1413
9. Severinov, K., Kashlev, M., Severinova, E., Bass, I., McWilliams, K., Kutter, E., Nikiforov, V., Snyder, L., and Goldfarb, A. (1994) A non-essential domain of E. coli RNA polymerase required for the action of the termination factor Alc. J. Biol. Chem., 269, 14254-14259
10. Severinov, K., Soushko, M., Goldfarb, A., and Nikiforov, V. (1994) RifR mutations in the beginning of Escherichia coli rpoB gene. Mol. Gen. Genet., 244, 120-126

Результаты исследований

Публикации

"Gelsolin-Like Activation of Villin: Calcium-Sensitivity of the Long Helix In Domain 6"
Stanislav O. Fedechkin , Jacob A. Brockerman , Danielle A. Pfaff , Lucian Burns , Terry Webb , Alexander Nelson , Fengli Zhang , Anton V Sabantsev, Alexey S Melnikov , Christopher James McKnight , Serge L. Smirnov
Biochemistry, 2013, V. 52, Is. 45, pp. 7890–7900

"Activity and bacterial diversity of snow around Russian Antarctic stations"
Anna Lopatina, Vjacheslav Krylenkov, Konstantin Severinov
Research in Microbiology, 2013, V. 164, Is. 9, pp. 949–958

"12-Fold symmetry of the putative portal protein from the Thermus thermophilus bacteriophage G20C determined by X-ray analysis"
Lowri S. Williams, Vladimir M. Levdikov, Leonid Minakhin, Konstantin Severinov and Alfred A. Antson
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 2013, V. 69, Is. 11, pp. 1239–1241

"Structure of Microcin B-Like Compounds Produced by Pseudomonas syringae and Species Specificity of Their Antibacterial Action"
Mikhail Metelev, Marina Serebryakova, Dmitry Ghilarov, Youfu Zhao, Konstantin Severinov
Journal of Bacteriology, 2013, V. 195, № 18, pp. 4129–4137

"Неканонические РНК-полимеразы гигантских бактериофагов типа φKZ"
М.В. Якунина, Д.Н. Воронцова, Л.С. Минахин
«Научно-технические ведомости СПбГПУ» серия «Физико-математические науки», 2013, № 1, с. 120-127


"Localization microscopy study of FtsZ structures in E. coli cells during SOS-response"
A D Vedyaykin, A V Sabantsev, I E Vishnyakov, S N Borchsenius, Y V Fedorova, A S Melnikov, P Yu Serdobintsev and M A Khodorkovskii
Journal of Physics: Conference Series, 2014, 541, 012036

"The RimL Transacetylase Provides Resistance to Translation Inhibitor Microcin C"
Teymur Kazakov, Konstantin Kuznedelov, Ekaterina Semenova, Damir Mukhamedyarov, Kirill A. Datsenko, Anastasija Metlitskaya, Gaston H. Vondenhoff, Anton Tikhonov, Vinayak Agarwale, Satish Naire, Arthur Van Aerschotd and Konstantin Severinov
Journal of Bacteriology, V. 196, № 19, pp. 3377–3385

"The Molecular Mechanism of Aminopropylation of Peptide-Nucleotide Antibiotic Microcin C"
Alexey Kulikovsky, Marina Serebryakova, Olga Bantysh, Anastasia Metlitskaya , Sergei Borukhov , Konstantin Severinov, and Svetlana Dubiley
Journal of the American Chemical Society, 2014, V. 136, Is. 31, pp. 11168–11175

"Development of giant bacteriophage ФKZ is independent of the host transcription apparatus"
Ceyssens PJ, Minakhin L, Van den Bossche A, Yakunina M, Klimuk E, Blasdel B, De Smet J, Noben JP, Bläsi U, Severinov K, Lavigne R
Journal of Virology, 2014, pii: JVI.01347-14

"Lytic Water Dynamics Reveal Evolutionarily Conserved Mechanisms of ATP Hydrolysis by TIP49 AAA+ ATPases"
Arina Afanasyeva, Angela Hirtreiter, Anne Schreiber, Dina Grohmann, Georgii Pobegalov, Adam R. McKay,Irina Tsaneva, Michael Petukhov, Emmanuel Kas, Mikhail Grigoriev, and Finn Werner
Structure, 2014, 22, pp. 1–11

"Antibiotic streptolydigin requires noncatalytic Mg2+ for binding to RNA polymerase"
Zorov S, Yuzenkova Y, Nikiforov V, Severinov K, Zenkin N
Antimicrobial Agents and Chemotherapy, 2014, V. 58, Is. 3, pp. 1420-4

"Pervasive generation of oppositely oriented spacers during CRISPR adaptation"
Shmakov S, Savitskaya E, Semenova E, Logacheva MD, Datsenko KA, Severinov K
Nucleic Acids Research, 2014, V. 42, Is. 9, pp. 5907-16

"Enzymatic synthesis of bioinformatically predicted microcin C-like compounds encoded by diverse bacteria"
Bantysh O, Serebryakova M, Makarova KS, Dubiley S, Datsenko KA, Severinov K
MBio, 2014, V. 5, Is. 3, e01059-14

"A bacteriophage transcription regulator inhibits bacterial transcription initiation by p-factor displacement"
Bing Liu, Andrey Shadrin, Carol Sheppard, Vladimir Mekler, Yingqi Xu, Konstantin Severinov, Steve Matthews, and Sivaramesh Wigneshweraraj
Nucleic Acids Research, 2014, V. 42, Is. 7, pp. 4294–4305

"Molecular basis of RNA polymerase promoter specificity switch revealed through studies of Thermus bacteriophage transcription regulator"
Severinov K, Minakhin L, Sekine S, Lopatina A, Yokoyama S.
Bacteriophage, 2014, V. 4, e29399

"CRISPR-Cas: outstanding questions remain: Comment on “Diversity, evolution, and therapeutic applications of small RNAs in prokaryotic and eukaryotic immune systems” by Edwin L. Cooper and Nicola Overstreet"
K. Severinov
Physics of Life Reviews, 2014, V. 11, Is. 1, pp. 146–148

"Structural basis for promoter specificity switching of RNA polymerase by a phage factor"
Shunsuke Tagami, Shun-ichi Sekine, Leonid Minakhin, Daria Esyunina, Ryogo Akasaka, Mikako Shirouzu, Andrey Kulbachinskiy, Konstantin Severinov, Shigeyuki Yokoyama
Genes & Development, 2014, V. 28, pp. 521-531

"The sabotage of the bacterial transcription machinery by a small bacteriophage protein"
Bing Liu, Andrey Shadrin, Carol Sheppard, Vladimir Mekler, Yingqi Xu, Konstantin Severinov, Steve Matthews, Sivaramesh Wigneshweraraj
Bacteriophage, 2014, V. 4, e28520

"The C-terminal part of microcin B is crucial for DNA gyrase inhibition and antibiotic uptake by sensitive cells"
Irina Shkundina, Marina Serebryakova, Konstantin Severinov
Journal of Bacteriology, 2014, V. 196, Is. 9, pp. 1759-1767


"Temporal dynamics of methyltransferase and restriction endonuclease accumulation in individual cells after introducing a restriction-modification system"
Natalia Morozova, Anton Sabantsev, Ekaterina Bogdanova, Yana Fedorova, Anna Maikova, Alexey Vedyaykin, Andjela Rodic, Marko Djordjevic, Mikhail Khodorkovskii and Konstantin Severinov
Nucleic Acids Research, 2015, doi: 10.1093/nar/gkv1490

"Enzymatic Synthesis and Functional Characterization of Bioactive Microcin C-Like Compounds with Altered Peptide Sequence and Length"
Olga Bantysh, Marina Serebryakova, Inna Zukher, Alexey Kulikovsky, Darya Tsibulskaya, Svetlana Dubiley, Konstantin Severinov
Journal of Bacteriology, 2015, 197, 19

"CRISPR interference and priming varies with individual spacer sequences"
Chaoyou Xue, Arun S. Seetharam, Olga Musharova, Konstantin Severinov, Stan J. J. Brouns, Andrew J. Severin and Dipali G. Sashital
Nucleic Acids Research, 2015, doi: 10.1093/nar/gkv1259

"Foreign DNA acquisition by the I-F CRISPR–Cas system requires all components of the interference machinery"
Daria Vorontsova, Kirill A. Datsenko, Sofia Medvedeva, Joseph Bondy-Denomy, Ekaterina E. Savitskaya, Ksenia Pougach, Maria Logacheva, Blake Wiedenheft, Alan R. Davidson, Konstantin Severinov, and Ekaterina Semenova
Nucleic Acids Research, 2015, doi: 10.1093/nar/gkv1261

"A non-canonical multisubunit RNA polymerase encoded by a giant bacteriophage"
Yakunina, Maria; Artamonova, Tatyana; Borukhov, Sergei; Severinov, Konstantin; Minakhin, Leonid
Nucleic Acids Research, 2015, doi: 10.1093/nar/gkv1095

"Structure, Bioactivity, and Resistance Mechanism of Streptomonomicin, an Unusual Lasso Peptide from an Understudied Halophilic Actinomycete"
Mikhail Metelev, Jonathan I. Tietz, Joel O. Melby, Patricia M. Blair, Lingyang Zhu, Itamar Livnat, Konstantin Severinov, Douglas A. Mitchell
Chemistry and Biology, 2015, Volume 22, Issue 2, p241–250

"The Cas6e ribonuclease is not required for interference and adaptation by the E. coli type I-E CRISPR-Cas system"
Semenova E, Kuznedelov K, Datsenko KA, Boudry PM, Savitskaya EE, Medvedeva S, Beloglazova N, Logacheva M, Yakunin AF, Severinov K
Nucleic Acids Research, 2015, doi: 10.1093/nar/gkv546

"Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection Through CRISPR"
Strotksaya, Alexandra; Semenova, Ekaterina; Savitskaya, Ekaterina; Severinov, Konstantin
Methods in molecular biology, 2015, V. 1311, pp. 147-159

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