Research Laboratory of Fluorescent Bioimaging
Grant agreement No.11.G34.31.0017 of November 24, 2010 was renewed on the basis of Paragraph 2 of Resolution of the Government of the Russian Federation No.220 of April 9, 2010, "On measures designed to attract the world's leading scientists to Russian institutions of higher learning, research institutions of the governmental academies of sciences and governmental research centers of the Russian Federation", and in compliance with the meeting minutes of the Grant Board of the Government of the Russian Federation established to provide governmental support to scientific research projects implemented under the supervision of the world's leading scientists at Russian institutions of higher learning, research institutions of the governmental academies of sciences and governmental research centers of the Russian Federation No.DL-1/16 of December 21, 2012.
Grant Agreement No.: 11.G34.31.0017
Host institution of higher learning:
State budget educational institution of higher professional education "Nizhniy Novgorod State Medical Academy" of the Ministry of health care of the Russian Federation"
Scientific research area:
To develop new approaches to researching the mechanisms of physiological and pathological processes in living systems.
Key project objectives:
1. To develop technologies allowing for observation of tumor cells in laboratory animals using fluorescent microscopy and tomography;
2. To research the possibilities of genetically encryptable photosensitizers for high-precision eradication of cancer cells in models using cellular lines and laboratory animals;
3. To analyze the dynamic of generation of active forms of oxygen in tumors using genetically encryptable hydrogen peroxide sensors;
4. To design methods of delivery of genetically encryptable markers, sensors, and photosensitizers to cancer cells in laboratory animals.
Anticipated project outputs:
The project will help create a phototoxic fluorescent protein capable of localizing in cellular nuclei and blocking cell divisions upon being exposed to green light. The protein is an effective tool that can be used to identify the role of certain cells in the processes of development, regeneration, and carcinogenesis in "in vivo" systems.
Leading scientist's full name: Lukyanov, Sergey Anatolyevich
Academic degree and title:
Doctor of biological sciences, member of the Russian Academy of Sciences.
Deputy Director for science of the Institute of Bioorganic Chemistry named after M. M. Shemyakin and Yu. A. Ovchinnikov; aid to the RF Miniser of health care
Field of scientific interests:
1. Genetic engineering.
2. Analysis of the structure and functions of eukaryotic genome.
Key scientific achievements:
- The leading scientist discovered the effect of selective suppression of the polymerase chain reaction and used it to design a number of new molecular-genetic methods that are now widely used to identify and analyze functionally important genetic sequences;
- He cloned the genes of fluorescent proteins in animals representing different systematic groups;
- He discovered non-fluorescent GFP-like chromoproteins that determine the color of many coral polyps;
- He discovered light-activated fluorescent proteins;
- He developed a genetically encodable photosensitizer;
- He created new fluorescent marking tools.
- Prize from the international academic publishing company "Science" for the best article published in its journals (1996);
- Winner of the "Bioorganic Chemistry" journal competition for the best article of the year (1996, 1997);
- His discovery of the fluorescent proteins of coral polyps was marked as one of the main achievements of the Russian Academy of Sciences in physical-chemical biology (1999);
- Academician Ovchinnikov Prize from the Presidium of the Russian Academy of Sciences for outstanding achievements in physical-chemical biology and biotechnology (2006);
- Author of more than 130 scientific works published in domestic and foreign periodicals;
- Author of 12 Russian and foreign patents;
- Regular lecturer at the department of molecular biology of the Faculty of biology of the Moscow State University named after M. V. Lomonosov.
- "Rusnanoprize" in medicine, pharmaceutics, and biotechnology for discovering and using fluorescent proteins in biotechnology (2012).
1. RF patent No.2458129. Isolated nucleic acid that encodes the KillerRed protein (variants), isolated protein (variants), expression cassette, cell containing the expression cassette. Authors: K. A. Lukyanov, E. O. Serebrovskaya, S. A. Lukyanov.
2. RF patent No.2493260. Isolated nucleic acid that encodes the fluorescent biosensor to detect hydrogen peroxide, expression cassette, biosensor-producing cell, isolated fluorescent biosensor, isolated nucleic acid that encodes the fluorescent biosensor that was fused with the nucleic acid that encodes the intracellular localization signal. Authors: D. S. Bilan, V. V. Belousov, S. A. Lukyanov.
3. RF patent No.2491342. Isolated nucleic acid that encodes the fused intramolecular KillerRed dimmer, expression cassette, the cell that produces chimeric protein and contains the expression cassette, isolated chimeric protein. Authors: K. A. Lukyanov, E. O. Serebrovskaya, S. A. Lukyanov.
4. RF patent No.2497121. Method of assessment of hydrogen peroxide on account of its presence in tumor cells as they are subjected to the action of an antitumor medication. Authors: A. S. Belova, V. V. Belousov, A. A. Brilkina, E. V. Zagainova, A. V. Maslennikova, N. M. Mishina, S. A. Lukyanov, A. G. Orlova, E. A. Sergeyeva, N. M. Shakhova.
5. Application for an RF invention patent No.2012112582, priority date: March 30, 2012. Isolated nucleic acid tat encodes a fluorescent biosensor, expression cassette, fluorescent biosensor-producing cell, isolated fluorescent biosensor. Authors: D. S. Bilan, V. V. Belousov, S. A. Lukyanov. Approved on November 22, 2013.
6. Application for an RF invention patent No.2012139381, priority date: September 13, 2012. A photodynamic tumor therapy method. Authors: M. V. Shirmanova, E. V. Zagainova, S. A. Lukyanov, E. O. Serebrovskaya, L. B. Snopova, M. L. Bugrova, E. A. Minakova, M. A. Sirotkina, V. A. Kamensky.
Grant agreement No.11.G34.31.0017 of November 24, 2010 (project implementation period: 2010 – 2012)
1. The project helped establish the Research Laboratory of Fluorescent Bioimaging that was fully equipped and made ready for implementation of research projects in the aforementioned field. The laboratory, established at the Nizhniy Novgorod State Medical Academy and spearheaded by the famous Russian academician S. A. Lukyanov, enlisted a team of 58 researchers, including one member of the Russian Academy of Sciences, one associate member of the Russian Academy of Sciences, eight doctors of sciences, 20 candidates of sciences, 15 graduate students, four young researchers, and nine undergraduate students. Sixteen of them, including eight doctors of sciences, five candidates of sciences, and three graduate students, are involved in teaching activities.
2. Over the course of the project, two graduate students were enrolled in the host university's graduate school from third party organizations (target: two graduate students).
3. Over the course of 2010 – 2012, the laboratory staff defended six term papers, four graduation diploma papers, and three candidate's degree dissertations on the subject of the research project in question.
4. Nineteen laboratory staff members underwent internships with some of the leading Russian and international research and education centers on the subject of the research project in question: seven of them were trained by representatives of foreign companies at the laboratory; four of them traveled abroad, and eight of them interned in Russia.
5. Over the course of 2010 – 2012, the laboratory staff members made 139 presentations at international conferences and 92 presentations at Russian conferences.
6. Over the course of 2010 – 2012, the laboratory organized, held, and hosted 35 events whereat 72 presentations were made on the subject of the research project in question.
7. Over the course of 2010 – 2012, the laboratory staff members published 66 articles on the subject of the research project in question in international peer review journals (with an aggregate impact factor of 156.42) that are referenced in the "Web of Science", "Scopus", and the Russian Scientific Citation Index. Nine of those articles had been written by the leading scientist in collaboration with the laboratory staff members. The aggregate impact factor of the articles written by the host institution's staff and faculty amounted to 0.2116.
8. Over the course of 2010 – 2012, the laboratory staff submitted six patent applications and received one invention patent (target: six patent applications).
9. The project staff initiated collaboration with 11 leading research and education institutions in Russia, as well as 12 universities from the USA, Germany, Great Britain, Switzerland, Australia, and the Netherlands.
10. The laboratory plans to become partially financially sustainable in the nearest future by making an ample use of its technological complex to perform preclinical tests of new diagnostic and therapeutic methods, tools, and medications. To that end, the laboratory has registered a small research-intensive business, "Molecular Technologies in Diagnostics of Cancer" (on the basis of RF Federal Law No.217-FZ of August 2, 2009), to implement the project results in biomedicine and commercialize its newly developed biotechnologies.
11. Over the course of 2010 – 2012, the laboratory offered four (target: four) courses of training on the subject of the research project in question. The number of young scientists (specialists, instructors) from third party organizations who underwent professional retraining or improved their professional qualifications at those courses amounted to 218 (target: 136).
12. The project scientists developed three special courses of training in biological engineering, optical bioimaging, and fluorescent proteins in medicine for the fifth and sixth year students, interns, and clinical residents. These special courses were personally delivered by lecturers invited from partner organizations (target: three special courses). The scientific results of the project were incorporated into the process of education.
13. The project has made it possible for the host institution of higher learning to increase the share of young scientists in its faculty and staff by recruiting four young candidates of sciences (target: four) and three young doctors of sciences (target: three).
The share of young specialists and teachers under 30 years of age who do not have an academic degree in the host institution's faculty and staff amounted to 10.25 (target: 10.25).
The share of young scientists (candidates of sciences under 35 years of age and doctors of sciences under 40 years of age) in the host institution's faculty and staff amounted to 14.44 (target: 14.44).